Coding

Part:BBa_K5237996:Design

Designed by: Marik Mueller   Group: iGEM24_Heidelberg   (2024-09-30)


Staple subunit: SV40 NLS-dSpCas9-SV40 NLS


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 823
    Illegal PstI site found at 2245
    Illegal PstI site found at 2449
    Illegal PstI site found at 2479
    Illegal PstI site found at 3691
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 823
    Illegal PstI site found at 2245
    Illegal PstI site found at 2449
    Illegal PstI site found at 2479
    Illegal PstI site found at 3691
    Illegal NotI site found at 4213
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 284
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 823
    Illegal PstI site found at 2245
    Illegal PstI site found at 2449
    Illegal PstI site found at 2479
    Illegal PstI site found at 3691
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 823
    Illegal PstI site found at 2245
    Illegal PstI site found at 2449
    Illegal PstI site found at 2479
    Illegal PstI site found at 3691
    Illegal NgoMIV site found at 1111
    Illegal NgoMIV site found at 2215
    Illegal NgoMIV site found at 2288
    Illegal NgoMIV site found at 2773
    Illegal NgoMIV site found at 3682
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

References